Example of a BCYE agar plate with visible colonies of Legionella after... Download Scientific
BD BBL™ BCYE (Buffered Charcoal Yeast Extract) Agar is used for the isolation and cultivation of Legionella species. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below. a. Add 0.1 mL of a culture containing 30-300 CFU/0.1 mL to each plate and spread-inoculate using a sterile glass spreader.
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NCM4006 BCYE Growth Supplement NCM4005 BCYE Growth Supplement (without L-Cysteine) Precaution Refer to SDS Preparation 1. Suspend 38.5 grams of the medium in 1L of purified water. 2. Heat with frequent agitation and boil for one minute to completely dissolve the medium. 3. Autoclave at 110 C for 10 minutes. Cool to 45 - 50 C. 4.
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This study is a methodological examination of the steps and processes involved in setting up the Gojjam Lymphoedema Best Practice Trial (GoLBeT; ISRCTN67805210), a highly pragmatic clinical trial conducted in northern Ethiopia. Challenges to the trial and strategies used to deal with them were explored, together with reasons for delays.
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Author summary Podoconiosis is a disease of the lymphatic vessels of the lower extremities, caused by chronic exposure to irritant soils. It results in leg swelling, commonly complicated by acute dermatolymphangioadenitis (ADLA, or 'acute attacks'), characterised by severe pain, fever and disability. The Gojjam Lymphoedema Best Practice Trial (GoLBeT) trial is a successfully completed.
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BCYE Agar with GPVC (Cat. no. W169) contains glycine, vancomycin, polymyxin and cycloheximide for the greater inhibition of secondary microflora and further selective isolation of Legionella spp. BCYE Agar with CCVC (Cat. no. G170) contains cephalothin, colistin, vancomycin, and cycloheximide. This is based on the formulation of Bopp et al.(14.
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In GoLBeT, there was a much greater overlap of steps than in the Global Health Research Process Map, in which steps proceed in a sequential, stepwise manner. In GoLBeT, steps often ran alongside each other, and were frequently developed together, along with key documents. For example, the GoLBeT funding proposal was developed alongside the budget.