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Introduction. Polo-like kinase 1 (PLK1), a member of the Polo-like kinase (PLK) family, is an enzyme mainly involved in cell cycle progression , .PLKs consist of an N-terminal Ser/Thr kinase domain and a C-terminal regulatory Polo-box domain (PBD), which is characteristic for this family of kinases .The PBD is crucial for the ligand binding and subcellular localization of kinases .


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The polo-box domain (PBD) present in Plk1's C-terminal non-catalytic region has been shown to play a central role in targeting the N-terminal kinase domain of Plk1 to specific subcellular locations. Subsequent studies reveal that PBD binds to a phosphorylated motif generated by one of the two mechanisms - self-priming by Plk1 itself or non.


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Without Sec62, the translocation pore of Sec61 remains closed by the plug domain, rendering the channel inactive. We further show that the lateral gate of Sec61 must first be partially opened by interactions between Sec61 and Sec63 in cytosolic and luminal domains, a simultaneous disruption of which completely closes the channel.


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Polo-like kinases (Plks) are the key regulators of cell cycle progression, the members of which share a kinase domain and a polo-box domain (PBD) that serves as a protein-binding module. While Plk1 is a promising target for antitumor therapy, Plk2 is regarded as a tumor suppressor even though the two Plks commonly recognize the S-pS/T-P motif.


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Polo-like kinase 1 (PLK1) is a key regulator enzyme of cell cycle progression, which belongs to a kinase family named Polo-like kinases [1, 2].This family is characterized by a C-terminal domain termed Polo-box domain (PBD), which represents a regulatory domain for the function of the N-terminal Ser/Thr kinase domain [].PLK1 is activated during mitosis [].


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If this was the case, Glu86 would bind close to Asp611 and Asp631 from polo-box domain, and this could explain why a Dbf4-E86K peptide binds to the polo-box domain of Cdc5 with higher affinity.


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The polo-box domain: a molecular integrator of mitotic kinase cascades and polo-like kinase function. Cell Cycle. 2004; 3:128-31. doi: 10.4161/cc.3.2.660. [Google Scholar] Luo J, Emanuele MJ, Li D, Creighton CJ, Schlabach MR, Westbrook TF, Wong KK, Elledge SJ. A genome-wide RNAi screen identifies multiple synthetic lethal interactions with.

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