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Project Methods The primary objective is to determine by RT-PCR methods if expression of genes associated with B cell differentiation occurs in skin of channel catfish and if infection with Ichthyophthirius multifiliis alters their expression. Originally, in vitro infection of skin explants was proposed, but a alternate approach will be used, which involves direct tail fin infection of channel.
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The pig is a logical choice to develop stem cells as the development of porcine stem cells offers significant opportunities to perform complex genetic modifications agricultural purposes, to study stem cell based therapies in large animal models and potentially eliminates the need for animal cloning for agricultural purposes. Similarities between human and porcine anatomy and physiology have.
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Very little is currently known about the immunity of fish skin against natural infection. Understanding the basic mechanisms of cutaneous immunity is critical, however, for development of efficacious vaccines and adjuvants for use against economically important pathogens, especially those that invade through epithelial surfaces such as the protozoan parasite Ichthyophthirius multifiliis.